Super-resolution 3D Microscopy of Whole Cells Opens New Window for Scientists

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Super-resolution 3D Microscopy of Whole Cells Opens New Window for Scientists

Super-resolution 3D Microscopy of Whole Cells Opens New Window for Scientists


Yale University researchers have developed a new Microscopy technique that allows them to have a 3D view of the insides of entire cells.

Dubbed*whole-cell 4Pi single-molecule switching na**scopy (W-4PiSMSN), the method improves on a technique called 4Pi that uses two objectives to provide a 3D version of Super-resolution microscopy. It allows researchers to pick specific structures to look at and the team that developed the tech**logy has already visualized the endoplasmic reticulum, bacteriophages, mitochondria, nuclear pore complexes, primary cilia, Golgi-apparatus-associated COPI vesicles, and mouse spermatocyte synaptonemal complexes.

Here’s a video produced by the Microscopy technique of 19 mouse chromosomes:



Study in journal Cell: Ultra-High Resolution 3D Imaging of Whole Cells…

More from Yale…

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