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06-13-2015, 04:13 AM
Three New Chemically Activated Fluorescent Compounds to Study Biological Systems
http://cdn.medgadget.com/wp-content/uploads/2015/06/multicolored-cell-markers.png
Fluorescent proteins, including the famous and commonly used green fluorescent protein (GFP), require an external light beam to strike the molecule for it to fluoresce. In the past, scientists have tried using luminescent proteins that are activated by other means, but their light was too dim and made them hard to see.***w Japanese researchers have developed so called “na**-lanterns,” luminescent proteins that light up in response to chemical changes rather than light, that are bright and produce different colored spectrum of light. This capability may be a huge benefit for biomedical researchers, allowing them to track all sorts of complicated mechanisms within the human body.
http://cdn.medgadget.com/wp-content/uploads/2015/06/sea-pansy.pngSea pansy. Photo credit – Gregory Badon
The research team from*RIKEN Quantitative Biology Center and*Osaka University used the sea pansy that produces the*Renilla luciferase enzyme (named after Lucifer, the bearer*of light) that*reacts with and activates*coelenterazine, a light emitting molecule. Under natural circumstances, this process results in a dim blue light that’s **t very useful for researchers, but the researchers used a genetically altered form of the enzyme to couple it with three different colored fluorescent proteins. This resulted in thee luminescent compounds that can be used individually or together to follow different biochemical processes.
Conveniently, the light emitted by the new compounds is bright e**ugh to be detected by a smartphone camera, which means that researchers can use them in situations where powerful microscopes are impractical or impossible.
Study in PNAS: Expanded palette of Na**-lanterns for real-time multicolor luminescence imaging… (http://www.pnas.org/content/112/14/4352)
Source: RIKEN… (http://www.riken.jp/en/research/rikenresearch/highlights/8036/)
The post Three New Chemically Activated Fluorescent Compounds to Study Biological Systems (http://www.medgadget.com/2015/06/three-new-chemically-activated-fluorescent-compounds-to-study-biological-systems.html) appeared first on Medgadget (http://www.medgadget.com).
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http://cdn.medgadget.com/wp-content/uploads/2015/06/multicolored-cell-markers.png
Fluorescent proteins, including the famous and commonly used green fluorescent protein (GFP), require an external light beam to strike the molecule for it to fluoresce. In the past, scientists have tried using luminescent proteins that are activated by other means, but their light was too dim and made them hard to see.***w Japanese researchers have developed so called “na**-lanterns,” luminescent proteins that light up in response to chemical changes rather than light, that are bright and produce different colored spectrum of light. This capability may be a huge benefit for biomedical researchers, allowing them to track all sorts of complicated mechanisms within the human body.
http://cdn.medgadget.com/wp-content/uploads/2015/06/sea-pansy.pngSea pansy. Photo credit – Gregory Badon
The research team from*RIKEN Quantitative Biology Center and*Osaka University used the sea pansy that produces the*Renilla luciferase enzyme (named after Lucifer, the bearer*of light) that*reacts with and activates*coelenterazine, a light emitting molecule. Under natural circumstances, this process results in a dim blue light that’s **t very useful for researchers, but the researchers used a genetically altered form of the enzyme to couple it with three different colored fluorescent proteins. This resulted in thee luminescent compounds that can be used individually or together to follow different biochemical processes.
Conveniently, the light emitted by the new compounds is bright e**ugh to be detected by a smartphone camera, which means that researchers can use them in situations where powerful microscopes are impractical or impossible.
Study in PNAS: Expanded palette of Na**-lanterns for real-time multicolor luminescence imaging… (http://www.pnas.org/content/112/14/4352)
Source: RIKEN… (http://www.riken.jp/en/research/rikenresearch/highlights/8036/)
The post Three New Chemically Activated Fluorescent Compounds to Study Biological Systems (http://www.medgadget.com/2015/06/three-new-chemically-activated-fluorescent-compounds-to-study-biological-systems.html) appeared first on Medgadget (http://www.medgadget.com).
http://feeds.feedburner.com/~ff/Medgadget?d=yIl2AUoC8zA (http://feeds.feedburner.com/~ff/Medgadget?a=3ofXwEYoNA0:RlLa6NM6LeI:yIl2AUoC8zA) http://feeds.feedburner.com/~ff/Medgadget?d=qj6IDK7rITs (http://feeds.feedburner.com/~ff/Medgadget?a=3ofXwEYoNA0:RlLa6NM6LeI:qj6IDK7rITs) http://feeds.feedburner.com/~ff/Medgadget?i=3ofXwEYoNA0:RlLa6NM6LeI:gIN9vFwOqvQ (http://feeds.feedburner.com/~ff/Medgadget?a=3ofXwEYoNA0:RlLa6NM6LeI:gIN9vFwOqvQ)
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