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04-30-2015, 11:31 PM
Hold On Tight: Permanent Attachment of Proteins to Na**particles for Potential HIV Vaccine Therapy
http://cdn.medgadget.com/wp-content/uploads/2015/04/na**particles-for-hiv-vaccine.jpgThe image above illustrates how proteins (copper-colored coils) modified with polyhistidine-tags (green diamonds) can be attached to na**particles (red circle). Credit: Jonathan Lovell.
Researchers at the University of Buffalo have developed a simple chemical approach to permanently*attach proteins to na**particles, which could lead to the development of a new HIV vaccine. While*na**particle-based delivery of proteins to areas of interest such as tumors is **t uncommon, this*method is a significant improvement from existing tech**logy, due to the “velcro” chemistry that*tightly binds the proteins to the na**particles. The research, recently published in the journal Nature*Chemistry, involves the use of na**particles called liposomes (made from phospholipids, which make*up the cell membrane) incorporated with chlorophyll (the pigment that makes leaves look green) and*cobalt (a metal used in batteries and dental prosthetics).
The proteins are modified with a chain of*ami** acids, called a poyhistidine tag. When the proteins and na**particles are mixed in water, the*chain tightly embeds into the outer layer of the na**particle, with the rest of the protein sticking out*like tentacles (pictured). When this system was tested with a vaccine delivery agent (adjuvant) and a*HIV peptide, it triggered a strong immune response against HIV. Considering how hard it is to find a*vaccination for HIV (http://www.aidsmap.com/Why-is-it-so-hard-to-make-a-vaccine-against-HIV/page/1271042), the potential for this research to turn into an actual therapy is quite tangible.
Study in Nature Chemistry: Functionalization of cobalt porphyrin–phospholipid bilayers with his-tagged ligands and antigens… (http://www.nature.com/nchem/journal/v7/n5/full/nchem.2236.html)
Source: University at Buffalo… (http://www.buffalo.edu/news/releases/2015/04/051.html)
The post Hold On Tight: Permanent Attachment of Proteins to Na**particles for Potential HIV Vaccine Therapy (http://www.medgadget.com/2015/04/hold-tight-permanent-attachment-proteins-na**particles-potential-hiv-vaccine-therapy.html) appeared first on Medgadget (http://www.medgadget.com).
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http://cdn.medgadget.com/wp-content/uploads/2015/04/na**particles-for-hiv-vaccine.jpgThe image above illustrates how proteins (copper-colored coils) modified with polyhistidine-tags (green diamonds) can be attached to na**particles (red circle). Credit: Jonathan Lovell.
Researchers at the University of Buffalo have developed a simple chemical approach to permanently*attach proteins to na**particles, which could lead to the development of a new HIV vaccine. While*na**particle-based delivery of proteins to areas of interest such as tumors is **t uncommon, this*method is a significant improvement from existing tech**logy, due to the “velcro” chemistry that*tightly binds the proteins to the na**particles. The research, recently published in the journal Nature*Chemistry, involves the use of na**particles called liposomes (made from phospholipids, which make*up the cell membrane) incorporated with chlorophyll (the pigment that makes leaves look green) and*cobalt (a metal used in batteries and dental prosthetics).
The proteins are modified with a chain of*ami** acids, called a poyhistidine tag. When the proteins and na**particles are mixed in water, the*chain tightly embeds into the outer layer of the na**particle, with the rest of the protein sticking out*like tentacles (pictured). When this system was tested with a vaccine delivery agent (adjuvant) and a*HIV peptide, it triggered a strong immune response against HIV. Considering how hard it is to find a*vaccination for HIV (http://www.aidsmap.com/Why-is-it-so-hard-to-make-a-vaccine-against-HIV/page/1271042), the potential for this research to turn into an actual therapy is quite tangible.
Study in Nature Chemistry: Functionalization of cobalt porphyrin–phospholipid bilayers with his-tagged ligands and antigens… (http://www.nature.com/nchem/journal/v7/n5/full/nchem.2236.html)
Source: University at Buffalo… (http://www.buffalo.edu/news/releases/2015/04/051.html)
The post Hold On Tight: Permanent Attachment of Proteins to Na**particles for Potential HIV Vaccine Therapy (http://www.medgadget.com/2015/04/hold-tight-permanent-attachment-proteins-na**particles-potential-hiv-vaccine-therapy.html) appeared first on Medgadget (http://www.medgadget.com).
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