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04-10-2015, 02:06 AM
New Device Delivers Thousands of Large Molecules Into Cells
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Injecting large molecules and even bacteria into the interior of mammalian cells has been a challenging and slow process. It takes about one minute by conventional methods*to inject one cell, but researchers at UCLA have developed a device that can do the same at an astonishing rate of 100,000 per minute.
While traditional techniques use tiny syringe-like pipettes, the new system, called biophotonic laser-assisted surgery tool (BLAST), uses a totally different approach. Cells are positioned on top of a silicon chip that has a large number of tiny holes throughout. The titanium coating of the chip is heated up using a laser, causing liquid below the holes to bubble and propel into the cell membrane, causing a small rupture. This happens in a tiny fraction of a second, letting particles within the liquid below the holes to enter the cells.
From the study abstract in Nature Methods:
The platform delivers large items including bacteria, enzymes, antibodies and na**particles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle ofFrancisella **vicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.
Study in Nature Methods: Massively parallel delivery of large cargo into mammalian cells with light pulses… (http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3357.html)
More from UCLA… (http://newsroom.ucla.edu/releases/ucla-researchers-deliver-large-particles-into-cells-at-high-speed)
The post New Device Delivers Thousands of Large Molecules Into Cells (http://www.medgadget.com/2015/04/new-device-delivers-thousands-large-molecules-cells.html) appeared first on Medgadget (http://www.medgadget.com).
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http://www.medgadget.com/wp-content/uploads/2015/04/large-particles-into-cells.jpg
Injecting large molecules and even bacteria into the interior of mammalian cells has been a challenging and slow process. It takes about one minute by conventional methods*to inject one cell, but researchers at UCLA have developed a device that can do the same at an astonishing rate of 100,000 per minute.
While traditional techniques use tiny syringe-like pipettes, the new system, called biophotonic laser-assisted surgery tool (BLAST), uses a totally different approach. Cells are positioned on top of a silicon chip that has a large number of tiny holes throughout. The titanium coating of the chip is heated up using a laser, causing liquid below the holes to bubble and propel into the cell membrane, causing a small rupture. This happens in a tiny fraction of a second, letting particles within the liquid below the holes to enter the cells.
From the study abstract in Nature Methods:
The platform delivers large items including bacteria, enzymes, antibodies and na**particles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle ofFrancisella **vicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.
Study in Nature Methods: Massively parallel delivery of large cargo into mammalian cells with light pulses… (http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3357.html)
More from UCLA… (http://newsroom.ucla.edu/releases/ucla-researchers-deliver-large-particles-into-cells-at-high-speed)
The post New Device Delivers Thousands of Large Molecules Into Cells (http://www.medgadget.com/2015/04/new-device-delivers-thousands-large-molecules-cells.html) appeared first on Medgadget (http://www.medgadget.com).
http://feeds.feedburner.com/~ff/Medgadget?d=yIl2AUoC8zA (http://feeds.feedburner.com/~ff/Medgadget?a=nHcjVFlUsYI:p0diPigCvRE:yIl2AUoC8zA) http://feeds.feedburner.com/~ff/Medgadget?d=qj6IDK7rITs (http://feeds.feedburner.com/~ff/Medgadget?a=nHcjVFlUsYI:p0diPigCvRE:qj6IDK7rITs) http://feeds.feedburner.com/~ff/Medgadget?i=nHcjVFlUsYI:p0diPigCvRE:gIN9vFwOqvQ (http://feeds.feedburner.com/~ff/Medgadget?a=nHcjVFlUsYI:p0diPigCvRE:gIN9vFwOqvQ)
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