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03-27-2015, 08:51 PM
Na**pore DNA Sequencing and Raspberry Pi Combine for Real-Time Environmental Studies
http://www.medgadget.com/wp-content/uploads/2015/03/MINION.jpgThe Ge**me Analysis Centre (TGAC) in **rwich, England has been using a na**pore-sensing tech**logy called the MinION (http://www.medgadget.com/2012/02/usb-powered-dna-na**pore-sequencing-for-900.html), a product introduced by Oxford Na**pore Tech**logies Ltd, a U.K.-based startup company. The research team plans to use this miniature device to develop a robust protocol for real-time DNA sequencing in the field in contrast to taking samples*back*to the laboratory and analyzing them there. The group has already successfully sequenced twenty bacteria samples taken from the environment in real-time.*Due to its small size, the device is said to have a large error profile, however, the team has come up with various ways to counteract that weakness.
For example, the scientists have combined the use of MinION and a bioinformatics tool, Kontaminant, to accurately*identify species as the DNA is being analyzed by MinION. Furthermore, to address the error profile of the device, researchers at TGAC have also written*a program called Na**OK that allows the user to analyze large amounts of data that are*collected by the MinION and compare them to reference DNA sequences for even greater accuracy of*identification. With these various tech**logies, the researchers were able to show that **t only was this process viable, but also that the samples taken from the environment*are able to be analyzed by the MinION and Kontaminant while being run on a low powered computer such as a*Raspberry Pi.
From the press release:
The ability to deploy real time monitoring in the field could change agriculture methods and how we look at crop disease outbreaks, as well as human disease epidemics. These are early days, but it looks like a combination of a small low power sequencing device with the correct experimental and computational approaches can spot pathogens,” said Dr Matt Clark, head of the MAP task force and Plant and Microbial Ge**mics Group Leader at TGAC.
Flashback:*USB-Powered DNA Na**pore Sequencing for $900 (http://www.medgadget.com/2012/02/usb-powered-dna-na**pore-sequencing-for-900.html)
TGAC press release: TGAC’s Take On First Portable DNA Sequency “Laboratory” (http://www.tgac.ac.uk/news/184/68/TGAC-s-take-on-the-first-portable-DNA-sequencing-laboratory/)
The post Na**pore DNA Sequencing and Raspberry Pi Combine for Real-Time Environmental Studies (http://www.medgadget.com/2015/03/na**pore-dna-sequencing-and-raspberry-pi-combine-for-real-time-environmental-studies.html) appeared first on Medgadget (http://www.medgadget.com).
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http://www.medgadget.com/wp-content/uploads/2015/03/MINION.jpgThe Ge**me Analysis Centre (TGAC) in **rwich, England has been using a na**pore-sensing tech**logy called the MinION (http://www.medgadget.com/2012/02/usb-powered-dna-na**pore-sequencing-for-900.html), a product introduced by Oxford Na**pore Tech**logies Ltd, a U.K.-based startup company. The research team plans to use this miniature device to develop a robust protocol for real-time DNA sequencing in the field in contrast to taking samples*back*to the laboratory and analyzing them there. The group has already successfully sequenced twenty bacteria samples taken from the environment in real-time.*Due to its small size, the device is said to have a large error profile, however, the team has come up with various ways to counteract that weakness.
For example, the scientists have combined the use of MinION and a bioinformatics tool, Kontaminant, to accurately*identify species as the DNA is being analyzed by MinION. Furthermore, to address the error profile of the device, researchers at TGAC have also written*a program called Na**OK that allows the user to analyze large amounts of data that are*collected by the MinION and compare them to reference DNA sequences for even greater accuracy of*identification. With these various tech**logies, the researchers were able to show that **t only was this process viable, but also that the samples taken from the environment*are able to be analyzed by the MinION and Kontaminant while being run on a low powered computer such as a*Raspberry Pi.
From the press release:
The ability to deploy real time monitoring in the field could change agriculture methods and how we look at crop disease outbreaks, as well as human disease epidemics. These are early days, but it looks like a combination of a small low power sequencing device with the correct experimental and computational approaches can spot pathogens,” said Dr Matt Clark, head of the MAP task force and Plant and Microbial Ge**mics Group Leader at TGAC.
Flashback:*USB-Powered DNA Na**pore Sequencing for $900 (http://www.medgadget.com/2012/02/usb-powered-dna-na**pore-sequencing-for-900.html)
TGAC press release: TGAC’s Take On First Portable DNA Sequency “Laboratory” (http://www.tgac.ac.uk/news/184/68/TGAC-s-take-on-the-first-portable-DNA-sequencing-laboratory/)
The post Na**pore DNA Sequencing and Raspberry Pi Combine for Real-Time Environmental Studies (http://www.medgadget.com/2015/03/na**pore-dna-sequencing-and-raspberry-pi-combine-for-real-time-environmental-studies.html) appeared first on Medgadget (http://www.medgadget.com).
http://feeds.feedburner.com/~ff/Medgadget?d=yIl2AUoC8zA (http://feeds.feedburner.com/~ff/Medgadget?a=8EeUZLHSpnY:BiPqBAB4PyU:yIl2AUoC8zA) http://feeds.feedburner.com/~ff/Medgadget?d=qj6IDK7rITs (http://feeds.feedburner.com/~ff/Medgadget?a=8EeUZLHSpnY:BiPqBAB4PyU:qj6IDK7rITs) http://feeds.feedburner.com/~ff/Medgadget?i=8EeUZLHSpnY:BiPqBAB4PyU:gIN9vFwOqvQ (http://feeds.feedburner.com/~ff/Medgadget?a=8EeUZLHSpnY:BiPqBAB4PyU:gIN9vFwOqvQ)
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